English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT

Released

Journal Article

Emergence of functional subnetworks in layer 2/3 cortex induced by sequential spikes in vivo

MPS-Authors

Kim,  Taekeun
Max Planck Florida Institute for Neuroscience, Max Planck Society;

Oh,  Won Chan
Max Planck Florida Institute for Neuroscience, Max Planck Society;

Choi,  Joon Ho
Max Planck Florida Institute for Neuroscience, Max Planck Society;

Kwon,  Hyung-Bae
Max Planck Florida Institute for Neuroscience, Max Planck Society;
MPI of Neurobiology, Max Planck Society;

External Ressource
No external resources are shared
Fulltext (public)
There are no public fulltexts stored in PuRe
Supplementary Material (public)
There is no public supplementary material available
Citation

Kim, T., Oh, W. C., Choi, J. H., & Kwon, H.-B. (2016). Emergence of functional subnetworks in layer 2/3 cortex induced by sequential spikes in vivo. Proceedings of the National Academy of Sciences, 113(10), 1372-1381. doi:10.1073/pnas.1513410113.


Cite as: http://hdl.handle.net/11858/00-001M-0000-002E-2C83-C
Abstract
During cortical circuit development in the mammalian brain, groups of excitatory neurons that receive similar sensory information form microcircuits. However, cellular mechanisms underlying cortical microcircuit development remain poorly understood. Here we implemented combined two-photon imaging and photolysis in vivo to monitor and manipulate neuronal activities to study the processes underlying activity-dependent circuit changes. We found that repeated triggering of spike trains in a randomly chosen group of layer 2/3 pyramidal neurons in the somatosensory cortex triggered long-term plasticity of circuits (LTPc), resulting in the increased probability that the selected neurons would fire when action potentials of individual neurons in the group were evoked. Significant firing pattern changes were observed more frequently in the selected group of neurons than in neighboring control neurons, and the induction was dependent on the time interval between spikes, N-methyl-D-aspartate (NMDA) receptor activation, and Calcium/calmodulin-dependent protein kinase II (CaMKII) activation. In addition, LTPc was associated with an increase of activity from a portion of neighboring neurons with different probabilities. Thus, our results demonstrate that the formation of functional microcircuits requires broad network changes and that its directionality is nonrandom, which may be a general feature of cortical circuit assembly in the mammalian cortex.