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Journal Article

Luciferases with tunable emission wavelengths

MPS-Authors
/persons/resource/persons212621

Hiblot,  Julien
Chemical Biology, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons212623

Yu,  Qiuliyang
Chemical Biology, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons212736

Reymond,  Luc
Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons212625

Xue,  Lin
Chemical Biology, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons203696

Johnsson,  Kai
Chemical Biology, Max Planck Institute for Medical Research, Max Planck Society;

External Resource

http://onlinelibrary.wiley.com/doi/10.1002/anie.201708277/epdf
(Any fulltext)

https://doi.org/10.1002/anie.201708277
(Any fulltext)

http://onlinelibrary.wiley.com/store/10.1002/anie.201708277/asset/supinfo/anie201708277-sup-0001-misc_information.pdf?v=1&s=d70b366e1364f37c721ed581bbde7b82d7fcf351
(Supplementary material)

http://onlinelibrary.wiley.com/store/10.1002/anie.201708277/asset/supinfo/anie201708277-sup-0001-Movie.avi?v=1&s=b3ad60cfe708a9065115bafdbb470855f840ea7c
(Supplementary material)

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Citation

Hiblot, J., Yu, Q., Sabbadini, M. D., Reymond, L., Xue, L., Schena, A., et al. (2017). Luciferases with tunable emission wavelengths. Angewandte Chemie, International Edition in English, 56(46), 14556-14560. doi:10.1002/anie.201708277.


Cite as: https://hdl.handle.net/11858/00-001M-0000-002E-2D2D-A
Abstract
We introduce luciferases whose emission maxima can be tuned to different wavelengths by chemical labeling. The luciferases are chimeras of NanoLuc with either SNAP-tag or HaloTag7. Labeling of the self-labeling tag with a fluorophore shifts the emission maximum of NanoLuc to that of the fluorophore. Luciferases with tunable colors have applications as reporter genes, for the construction of biosensors and in bioimaging.