Luciferases with tunable emission wavelengths

Hiblot, Julien; Yu, Qiuliyang; Sabbadini , Marina D.B.; Reymond, Luc; Xue, Lin; Schena , Alberto; Sallin , Olivier; Hill , Nicholas; Griss , Rudolf; Johnsson, Kai

doi:10.1002/anie.201708277

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Luciferases with tunable emission wavelengths

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Hiblot, Julien
Chemical Biology, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons212623

Yu, Qiuliyang
Chemical Biology, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons212736

Reymond, Luc
Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons212625

Xue, Lin
Chemical Biology, Max Planck Institute for Medical Research, Max Planck Society;

/persons/resource/persons203696

Johnsson, Kai
Chemical Biology, Max Planck Institute for Medical Research, Max Planck Society;

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引用

Hiblot, J., Yu, Q., Sabbadini, M. D., Reymond, L., Xue, L., Schena, A., Sallin, O., Hill, N., Griss, R., & Johnsson, K. (2017). Luciferases with tunable emission wavelengths. Angewandte Chemie, International Edition in English, 56(46), 14556-14560. doi:10.1002/anie.201708277.

引用: https://hdl.handle.net/11858/00-001M-0000-002E-2D2D-A

要旨

We introduce luciferases whose emission maxima can be tuned to different wavelengths by chemical labeling. The luciferases are chimeras of NanoLuc with either SNAP-tag or HaloTag7. Labeling of the self-labeling tag with a fluorophore shifts the emission maximum of NanoLuc to that of the fluorophore. Luciferases with tunable colors have applications as reporter genes, for the construction of biosensors and in bioimaging.