Structure of the recombinant antibody Fab fragment f3p4

Frey, Daniel; Huber, Thomas; Plückthun, Andreas; Grütter, Markus G.

doi:10.1107/S0907444908007282

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Structure of the recombinant antibody Fab fragment f3p4

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Frey, Daniel
Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society;

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http://journals.iucr.org/d/issues/2008/06/00/yt5005/yt5005.pdf
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Citation

Frey, D., Huber, T., Plückthun, A., & Grütter, M. G. (2008). Structure of the recombinant antibody Fab fragment f3p4. Acta Crystallographica Section D-Biological Crystallography, 64(6), 636-643. doi:10.1107/S0907444908007282.

Cite as: https://hdl.handle.net/11858/00-001M-0000-002E-8DCE-8

Abstract

The structure of the antibody Fab fragment f3p4, which was selected from a subset of the synthetic HuCAL antibody library to bind the sodium citrate symporter CitS, is described at 1.92 A resolution. Comparison with computational models revealed deviations in a few framework positions and in the binding loops. The crystals belong to space group P2(1)2(1)2 and contain four molecules in the asymmetric unit, with unit-cell parameters a=102.77, b=185.92, c=102.97 A. These particular unit-cell parameters allowed pseudo-merohedral twinning; interestingly, the twinning law relates a twofold screw axis to a twofold axis.