English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT

Released

Journal Article

Structure of the recombinant antibody Fab fragment f3p4

MPS-Authors
/persons/resource/persons117946

Frey,  Daniel
Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society;

Fulltext (restricted access)
There are currently no full texts shared for your IP range.
Fulltext (public)
There are no public fulltexts stored in PuRe
Supplementary Material (public)
There is no public supplementary material available
Citation

Frey, D., Huber, T., Plückthun, A., & Grütter, M. G. (2008). Structure of the recombinant antibody Fab fragment f3p4. Acta Crystallographica Section D-Biological Crystallography, 64(6), 636-643. doi:10.1107/S0907444908007282.


Cite as: https://hdl.handle.net/11858/00-001M-0000-002E-8DCE-8
Abstract
The structure of the antibody Fab fragment f3p4, which was selected from a subset of the synthetic HuCAL antibody library to bind the sodium citrate symporter CitS, is described at 1.92 A resolution. Comparison with computational models revealed deviations in a few framework positions and in the binding loops. The crystals belong to space group P2(1)2(1)2 and contain four molecules in the asymmetric unit, with unit-cell parameters a=102.77, b=185.92, c=102.97 A. These particular unit-cell parameters allowed pseudo-merohedral twinning; interestingly, the twinning law relates a twofold screw axis to a twofold axis.