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Journal Article

Cell-accurate optical mapping across the entire developing heart

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Scherf,  Nico
Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany;
Department Neurophysics (Weiskopf), MPI for Human Cognitive and Brain Sciences, Max Planck Society;

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Weber_Scherf_2017.pdf
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Citation

Weber, M., Scherf, N., Meyer, A. M., Panáková, D., Kohl, P., & Huisken, J. (2017). Cell-accurate optical mapping across the entire developing heart. eLife, 6: e28307. doi:10.7554/eLife.28307.001.


Cite as: http://hdl.handle.net/21.11116/0000-0000-27A9-6
Abstract
Organogenesis depends on orchestrated interactions between individual cells and morphogenetically relevant cues at the tissue level. This is true for the heart, whose function critically relies on well-ordered communication between neighboring cells, which is established and fine-tuned during embryonic development. For an integrated understanding of the development of structure and function, we need to move from isolated snap-shot observations of either microscopic or macroscopic parameters to simultaneous and, ideally continuous, cell-to-organ scale imaging. We introduce cell-accurate three-dimensional Ca2+-mapping of all cells in the entire electro-mechanically uncoupled heart during the looping stage of live embryonic zebrafish, using high-speed light sheet microscopy and tailored image processing and analysis. We show how myocardial region-specific heterogeneity in cell function emerges during early development and how structural patterning goes hand-in-hand with functional maturation of the entire heart. Our method opens the way to systematic, scale-bridging, in vivo studies of vertebrate organogenesis by cell-accurate structure-function mapping across entire organs.