English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT

Released

Journal Article

Strongly enhanced bacterial bioluminescence with the ilux operon for single-cell imaging.

MPS-Authors
/persons/resource/persons209303

Gregor,  C.
Department of NanoBiophotonics, MPI for Biophysical Chemistry, Max Planck Society;

/persons/resource/persons96528

Gwosch,  K.
Department of NanoBiophotonics, MPI for Biophysical Chemistry, Max Planck Society;

/persons/resource/persons15739

Sahl,  S. J.
Department of NanoBiophotonics, MPI for Biophysical Chemistry, Max Planck Society;

/persons/resource/persons15210

Hell,  S. W.
Department of NanoBiophotonics, MPI for Biophysical Chemistry, Max Planck Society;

External Ressource
No external resources are shared
Fulltext (public)

2525924.pdf
(Publisher version), 2MB

Supplementary Material (public)

2525924-Suppl.pdf
(Supplementary material), 2MB

Citation

Gregor, C., Gwosch, K., Sahl, S. J., & Hell, S. W. (2018). Strongly enhanced bacterial bioluminescence with the ilux operon for single-cell imaging. Proceedings of the National Academy of Sciences of the United States of America, 115(5), 962-967. doi:10.1073/pnas.1715946115.


Cite as: http://hdl.handle.net/21.11116/0000-0000-2E0D-0
Abstract
Bioluminescence imaging of single cells is often complicated by the requirement of exogenous luciferins that can be poorly cell-permeable or produce high background signal. Bacterial bioluminescence is unique in that it uses reduced flavin mononucleotide as a luciferin, which is abundant in all cells, making this system purely genetically encodable by the lux operon. Unfortunately, the use of bacterial bioluminescence has been limited by its low brightness compared with other luciferases. Here, we report the generation of an improved lux operon named ilux with an approximately sevenfold increased brightness when expressed in Escherichia coli; ilux can be used to image single E. coli cells with enhanced spatiotemporal resolution over several days. In addition, since only metabolically active cells produce bioluminescent signal, we show that ilux can be used to observe the effect of different antibiotics on cell viability on the single-cell level.