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Journal Article

The gonadotropin-releasing hormone associated peptide reduces calcium entry in prolactin-secreting cells

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Seeburg,  Peter H.
Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society;

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Citation

Vacher, P., Mariot, P., Dufy−Barbe, L., Nikolics, K., Seeburg, P. H., Seeburg, P. H., et al. (1991). The gonadotropin-releasing hormone associated peptide reduces calcium entry in prolactin-secreting cells. Endocrinology, 128(1), 285-294. doi:10.1210/endo-128-1-285.


Cite as: http://hdl.handle.net/21.11116/0000-0000-724C-B
Abstract
The precursor molecule to the GnRH contains a peptide named GnRH-associated peptide (GAP) with PRL-inhibiting properties. In this work, we have studied the electrophysiological properties and responses to GAP of three different types of PRL-secreting cells: 1) the rat tumor cell line GH3, 2) normal rat pituitary cells in primary culture, and 3) human PRL-secreting adenoma cells. Using different but complementary techniques we show that GAP reduces intracellular Ca++ levels, [Ca++]i, and inhibits Ca++ transients in these cells. This reduction of [Ca++]i results from coordinate actions of GAP on K+ and Ca++ conductances and may explain the inhibitory effect of GAP on hormonal secretion by PRL-secreting cells.