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Determining the bacterial cell biology of Planctomycetes

MPG-Autoren
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Reintjes,  Greta
Department of Molecular Ecology, Max Planck Institute for Marine Microbiology, Max Planck Society;

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Schaffer,  Miroslava
Baumeister, Wolfgang / Molecular Structural Biology, Max Planck Institute of Biochemistry, Max Planck Society;

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Amann,  Rudolf
Department of Molecular Ecology, Max Planck Institute for Marine Microbiology, Max Planck Society;

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Engelhardt,  Harald
Baumeister, Wolfgang / Molecular Structural Biology, Max Planck Institute of Biochemistry, Max Planck Society;

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Jogler,  Christian
Microbial Genomics Group, Department of Molecular Ecology, Max Planck Institute for Marine Microbiology, Max Planck Society;

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Zitation

Boedeker, C., Schueler, M., Reintjes, G., Jeske, O., van Teeseling, M. C. F., Jogler, M., et al. (2017). Determining the bacterial cell biology of Planctomycetes. NATURE COMMUNICATIONS, 8: 14853. doi:10.1038/ncomms14853.


Zitierlink: http://hdl.handle.net/21.11116/0000-0001-C1CF-C
Zusammenfassung
Bacteria of the phylum Planctomycetes have been previously reported to possess several features that are typical of eukaryotes, such as cytosolic compartmentalization and endocytosis-like macromolecule uptake. However, recent evidence points towards a Gram-negative cell plan for Planctomycetes, although in-depth experimental analysis has been hampered by insufficient genetic tools. Here we develop methods for expression of fluorescent proteins and for gene deletion in a model planctomycete, Planctopirus limnophila, to analyse its cell organization in detail. Super-resolution light microscopy of mutants, cryo-electron tomography, bioinformatic predictions and proteomic analyses support an altered Gram-negative cell plan for Planctomycetes, including a defined outer membrane, a periplasmic space that can be greatly enlarged and convoluted, and an energized cytoplasmic membrane. These conclusions are further supported by experiments performed with two other Planctomycetes, Gemmata obscuriglobus and Rhodopirellula baltica. We also provide experimental evidence that is inconsistent with endocytosis-like macromolecule uptake; instead, extracellular macromolecules can be taken up and accumulate in the periplasmic space through unclear mechanisms.