Covalent binding of BMP-2 on surfaces using a self-assembled monolayer approach

Pohl, Theresa; Schwab, Elisabeth; Cavalcanti-Adam, Elisabetta Ada

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Covalent binding of BMP-2 on surfaces using a self-assembled monolayer approach

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Pohl, Theresa
Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society;

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Schwab, Elisabeth
Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society;
Biophysical Chemistry, Institute of Physical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany;

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Cavalcanti-Adam, Elisabetta Ada
Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society;
Biophysical Chemistry, Institute of Physical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany;

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Pohl, T., Schwab, E., & Cavalcanti-Adam, E. A. (2013). Covalent binding of BMP-2 on surfaces using a self-assembled monolayer approach. Journal of visualized experiments.

Cite as: https://hdl.handle.net/21.11116/0000-0000-81AE-A

Abstract

We describe a method for accomplishing efficient immobilization of BMP-2 on surfaces. Our approach is based on the formation of a self-assembled monolayer to achieve the covalent binding of BMP-2 via its free amine residues. This method is a useful tool to study signaling at the cell membrane.