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GABAA receptor beta subunit heterogeneity: functional expression of cloned cDNAs

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Draguhn,  Andreas
Department of Cell Physiology, Max Planck Institute for Medical Research, Max Planck Society;

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Werner,  Pia
Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society;

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Köhler,  Martin
Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society;

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Seeburg,  Peter H.
Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society;

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Citation

Ymer, S., Schofield, P. R., Draguhn, A., Werner, P., Köhler, M., & Seeburg, P. H. (1989). GABAA receptor beta subunit heterogeneity: functional expression of cloned cDNAs. EMBO Journal, 8(6), 1665-1670. doi:10.1002/j.1460-2075.1989.tb03557.x.


Cite as: http://hdl.handle.net/21.11116/0000-0000-8E3B-F
Abstract
Cloned cDNAs encoding two new beta subunits of the rat and bovine GABAA receptor have been isolated using a degenerate oligonucleotide probe based on a highly conserved peptide sequence in the second transmembrane domain of GABAA receptor subunits. The beta 2 and beta 3 subunits share approximately 72% sequence identity with the previously characterized beta 1 polypeptide. Northern analysis showed that both beta 2 and beta 3 mRNAs are more abundant in the brain than beta 1 mRNA. All three beta subunit encoding cDNAs were also identified in a library constructed from adrenal medulla RNA. Each beta subunit, when co-expressed in Xenopus oocytes with an alpha subunit, forms functional GABAA receptors. These results, together with the known alpha subunit heterogeneity, suggest that a variety of related but functionally distinct GABAA receptor subtypes are generated by different subunit combinations.