Fast calcium transients in rat peritoneal mast cells are not sufficient to 
trigger exocytosis

Neher, E.; Almers, W.

doi:10.1002/j.1460-2075.1986.tb04176.x

Item

ITEM ACTIONSEXPORT

Add to Basket

Local TagsRelease HistoryDetailsSummary

Released

Journal Article

Fast calcium transients in rat peritoneal mast cells are not sufficient to trigger exocytosis

MPS-Authors

/persons/resource/persons15570

Neher, E.
Department of Membrane Biophysics, MPI for biophysical chemistry, Max Planck Society;

External Resource

No external resources are shared

Fulltext (restricted access)

There are currently no full texts shared for your IP range.

Fulltext (public)

There are no public fulltexts stored in PuRe

Supplementary Material (public)

There is no public supplementary material available

Citation

Neher, E., & Almers, W. (1986). Fast calcium transients in rat peritoneal mast cells are not sufficient to trigger exocytosis. EMBO Journal, 5(1), 51-53. doi:10.1002/j.1460-2075.1986.tb04176.x.

Cite as: https://hdl.handle.net/21.11116/0000-0000-B106-1

Abstract

The calcium concentration, [Ca]i, in single rat peritoneal mast cells was measured by means of the new Ca indicator dye fura-2. Upon stimulation with antigen or compound 48/80, [Ca]i rose for seconds to values greater than 5 microM. These Ca transients did not depend on the presence of extracellular Ca, and they sometimes occurred spontaneously, especially in the presence of exogenous phosphatidylserine. Calcium transients did not necessarily lead to degranulation. Degranulation usually occurred during periods of somewhat elevated [Ca] (0.5-1 microM) following transients but was sometimes observed at [Ca]i less than or equal to 250 nM. We found no evidence that an antigen-induced Ca influx is required for degranulation.