English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT

Released

Journal Article

Optogenetic Control of Ca2+ and Voltage-Dependent Large Conductance (BK) Potassium Channels

MPS-Authors
/persons/resource/persons137787

Mager,  Thomas
Department of Biophysical Chemistry, Max Planck Institute of Biophysics, Max Planck Society;

/persons/resource/persons137950

Wood,  Philip G.
Department of Biophysical Chemistry, Max Planck Institute of Biophysics, Max Planck Society;

/persons/resource/persons137592

Bamberg,  Ernst
Department of Biophysical Chemistry, Max Planck Institute of Biophysics, Max Planck Society;

Locator
There are no locators available
Fulltext (public)
There are no public fulltexts available
Supplementary Material (public)
There is no public supplementary material available
Citation

Mager, T., Wood, P. G., & Bamberg, E. (2017). Optogenetic Control of Ca2+ and Voltage-Dependent Large Conductance (BK) Potassium Channels. Journal of Molecular Biology (London), 429(6), 911-921. doi:10.1016/j.jmb.2017.02.004.


Cite as: http://hdl.handle.net/21.11116/0000-0001-27AF-F
Abstract
Ca2+ concentration jumps for the activation of Ca2+-dependent ion channels or transporters can be obtained either by fast solution exchange or by the use of caged Ca2+. Here, we report on an alternate optogenetic method for the activation of Ca2+ and voltage-dependent large conductance (BK) potassium channels. This was achieved through the use of the light-gated channelrhodopsin 2 variant, CatCh(Calcium translocating Channelrhodopsin) with enhanced Ca, which produces locally [Ca2+] in the μM range on the inner side of the membrane, without significant [Ca2+] increase in the cytosol. BK channel subunits α and β1 were expressed together with CatCh in HEK293 cells, and voltage and Ca2+ dependence were analyzed. Light activation of endogenous BK channels under native conditions in astrocytes and glioma cells was also investigated. Additionally, BK channels were used as sensors for the calibration of the [Ca2+] on the inner surface of the cell membrane.