Patch-clamp measurements of elementary ionic currents activated by the putative 
inhibitory transmitters GABA and glycine in mammalian spinal neurones

Sakmann, Bert; Hamill, Owen P.; Bormann, Jochen

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Patch-clamp measurements of elementary ionic currents activated by the putative inhibitory transmitters GABA and glycine in mammalian spinal neurones

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Sakmann, Bert
Department of Cell Physiology, Max Planck Institute for Medical Research, Max Planck Society;

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Citation

Sakmann, B., Hamill, O. P., & Bormann, J. (1983). Patch-clamp measurements of elementary ionic currents activated by the putative inhibitory transmitters GABA and glycine in mammalian spinal neurones. Journal of Neural Transmission-Supplement, 18, 83-95.

Cite as: https://hdl.handle.net/21.11116/0000-0000-ED94-E

Abstract

Methods for measuring whole-cell and unitary Cl- currents in the soma membrane of cultured spinal cord neurons by the patch-clamp technique are described. To separate single channel Cl- currents activated by the two putative inhibitory transmitters GABA and glycine from other membrane currents, isotonic KCl pipette solutions are used for measurements on cell-attached membrane patches. To isolate unitary Cl- currents in cell-free membrane patches, symmetrical, isotonic Tris Cl- solutions are used in both the pipette and the bath. Single Cl- channels are opened following binding of two agonist molecules to their respective receptors. The gating behavior of single receptor-channel complexes is described in terms of the activation and desensitization mechanism suggested originally by del Castillo and Katz (1957) and Katz and Thesleff (1957)