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Journal Article

A genome-wide resource for the analysis of protein localisation in Drosophila.

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Sarov,  Mihail
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

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Jambor,  Helena
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

Suchold,  Dana
Max Planck Society;

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Janosch,  Stephan
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

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Hasse,  Susanne
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

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Knust,  Elisabeth
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

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Tomancak,  Pavel
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

Schnorrer,  Frank
Max Planck Society;

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Citation

Sarov, M., Barz, C., Jambor, H., Hein, M. Y., Schmied, C., Suchold, D., et al. (2016). A genome-wide resource for the analysis of protein localisation in Drosophila. eLife, 5: e12068.


Cite as: http://hdl.handle.net/21.11116/0000-0001-0268-8
Abstract
The Drosophila genome contains >13,000 protein coding genes, the majority of which remain poorly investigated. Important reasons include the lack of antibodies or reporter constructs to visualise these proteins. Here we present a genome-wide fosmid library of 10,000 GFP-tagged clones, comprising tagged genes and most of their regulatory information. For 880 tagged proteins we created transgenic lines and for a total of 207 lines we assessed protein expression and localisation in ovaries, embryos, pupae or adults by stainings and live imaging approaches. Importantly, we visualised many proteins at endogenous expression levels and found a large fraction of them localising to subcellular compartments. By applying genetic complementation tests we estimate that about two-thirds of the tagged proteins are functional. Moreover, these tagged proteins enable interaction proteomics from developing pupae and adult flies. Taken together, this resource will boost systematic analysis of protein expression and localisation in various cellular and developmental contexts.