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Journal Article

Optotaxis: Caged Lysophosphatidic Acid Enables Optical Control of a Chemotactic Gradient.

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Nadler,  André
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

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Citation

Hövelmann, F., Kedziora, K. M., Nadler, A., Müller, R., Jalink, K., & Schultz, C. (2016). Optotaxis: Caged Lysophosphatidic Acid Enables Optical Control of a Chemotactic Gradient. Cell Chemical Biology, 23(5), 629-634.


Cite as: https://hdl.handle.net/21.11116/0000-0001-032B-C
Abstract
Lysophosphatidic acid (LPA) is a serum-borne lipid mediator that binds to a variety of different G protein-coupled receptors to trigger an exceptionally wide range of biological effects, including cell survival and differentiation, cancer cell migration, and embryonic development. Here we synthesized caged LPA (cgLPA), a "photolysable" coumarin-masked derivative of LPA. We demonstrate that illumination of cgLPA with 405 nm light liberates bioactive LPA on a subsecond scale to evoke Ca(2+) signaling, Rho activation, and cytoskeletal contraction. In addition, we developed an "optotaxis" assay to attract melanoma cells through a stable chemotactic gradient by repeated liberation of LPA through local photolysis of extracellular cgLPA. We expect that this method of light-controlled chemotaxis will be generally applicable to a large variety of small molecules that drive cellular migration or other responses.