English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
Add to Basket
  Local TagsRelease HistoryDetailsSummary

Released
Journal Article

Clonal expansion capacity defines two consecutive developmental stages of long-term hematopoietic stem cells.

MPS-Authors

Portz,  Melanie
Max Planck Society;

/persons/resource/persons219366

Lakshmanaperumal,  Naharajan
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

/persons/resource/persons219236

Henry,  Ian
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

Dahl,  Andreas
Max Planck Society;

Waskow,  Claudia
Max Planck Society;

External Resource
No external resources are shared
Fulltext (restricted access)
There are currently no full texts shared for your IP range.
Fulltext (public)
There are no public fulltexts stored in PuRe
Supplementary Material (public)
There is no public supplementary material available
Citation

Grinenko, T., Arndt, K., Portz, M., Mende, N., Günther, M., Cosgun, K. N., et al. (2014). Clonal expansion capacity defines two consecutive developmental stages of long-term hematopoietic stem cells. The Journal of Experimental Medicine, 211(2), 209-215.


Cite as: https://hdl.handle.net/21.11116/0000-0001-0550-F
Abstract
Long-term hematopoietic stem cells (HSCs [LT-HSCs]) are well known to display unpredictable differences in their clonal expansion capacities after transplantation. Here, by analyzing the cellular output after transplantation of stem cells differing in surface expression levels of the Kit receptor, we show that LT-HSCs can be systematically subdivided into two subtypes with distinct reconstitution behavior. LT-HSCs expressing intermediate levels of Kit receptor (Kit(int)) are quiescent in situ but proliferate extensively after transplantation and therefore repopulate large parts of the recipient's hematopoietic system. In contrast, metabolically active Kit(hi) LT-HSCs display more limited expansion capacities and show reduced but robust levels of repopulation after transfer. Transplantation into secondary and tertiary recipient mice show maintenance of efficient repopulation capacities of Kit(int) but not of Kit(hi) LT-HSCs. Initiation of differentiation is marked by the transit from Kit(int) to Kit(hi) HSCs, both of which precede any other known stem cell population.