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Journal Article

High-resolution reconstruction of the beating zebrafish heart.

MPS-Authors
/persons/resource/persons219450

Mickoleit,  Michaela
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

/persons/resource/persons219633

Schmid,  Benjamin
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

/persons/resource/persons219779

Weber,  Michael
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

/persons/resource/persons219147

Fahrbach,  Florian
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

/persons/resource/persons219245

Hombach,  Sonja
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

/persons/resource/persons219250

Huisken,  Jan
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

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Citation

Mickoleit, M., Schmid, B., Weber, M., Fahrbach, F., Hombach, S., Reischauer, S., et al. (2014). High-resolution reconstruction of the beating zebrafish heart. Nature Methods, 11(9), 919-922.


Cite as: https://hdl.handle.net/21.11116/0000-0001-058E-A
Abstract
The heart's continuous motion makes it difficult to capture high-resolution images of this organ in vivo. We developed tools based on high-speed selective plane illumination microscopy (SPIM), offering pristine views into the beating zebrafish heart. We captured three-dimensional cardiac dynamics with postacquisition synchronization of multiview movie stacks, obtained static high-resolution reconstructions by briefly stopping the heart with optogenetics and resolved nonperiodic phenomena by high-speed volume scanning with a liquid lens.