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Journal Article

OpenSPIM: an open access light sheet microscopy platform

MPS-Authors
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Pitrone,  Peter Gabriel
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

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Schindelin,  Johannes
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

Stuyvenberg,  Luke
Max Planck Society;

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Preibisch,  Stephan W.
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

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Weber,  Michael
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

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Huisken,  Jan
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

/persons/resource/persons219742

Tomancak,  Pavel
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

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Citation

Pitrone, P. G., Schindelin, J., Stuyvenberg, L., Preibisch, S. W., Weber, M., Eliceiri, K. W., et al. (2013). OpenSPIM: an open access light sheet microscopy platform. Nature Methods, 10(7), 598-599.


Cite as: https://hdl.handle.net/21.11116/0000-0001-0718-D
Abstract
Light-sheet microscopy is revolutionizing biology by enabling live in toto imaging of entire embryos or organs with minimal phototoxicity1. We present an open hardware and software platform for constructing a customizable microscope for selective- plane illumination microscopy (SPIM). The OpenSPIM platform is shared with the scientific community through a public website (http://openspim.org/), making light-sheet microscopy more acces- sible so that it can be optimized for various applications.