The human kinesin-14 HSET tracks the tips of growing microtubules in vitro.

Braun, Marcus; Lansky, Zdenek; Bajer, Seweryn; Fink, Gero; Kasprzak, Andrzej A; Diez, Stefan

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The human kinesin-14 HSET tracks the tips of growing microtubules in vitro.

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Braun, Marcus
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

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Lansky, Zdenek
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

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Fink, Gero
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

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Diez, Stefan
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

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引用

Braun, M., Lansky, Z., Bajer, S., Fink, G., Kasprzak, A. A., & Diez, S. (2013). The human kinesin-14 HSET tracks the tips of growing microtubules in vitro. Cytoskeleton (Hoboken, N.J.), 70(9), 515-521.

引用: https://hdl.handle.net/21.11116/0000-0001-0768-3

要旨

Tip-tracking of kinesin-14 motor proteins is believed to be crucial for the assembly and maintenance of dynamic microtubule arrays. However, in contrast to other members of the kinesin-14 family, H. sapiens kinesin-14 HSET has so far never been observed to be prominently located at microtubule plus ends. Here, using an in vitro microtubule dynamics reconstitution assay we observe tip-tracking of GFP-HSET in the presence of H. sapiens EB1 (hsEB1). Tip-tracking depended on the SxIP-like motif in HSET as well as on the EB homology domain in hsEB1. D. melanogaster Ncd and S. pombe Klp2 tip-tracking reconstitution assays accompanied by kinesin-14 amino acid sequence comparisons suggest that SxIP-like motif mediated tip-tracking dependent on EB family proteins is conserved in the kinesin-14 family of molecular motors.