Slicing embryos gently with laser light sheets.

Huisken, Jan

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Slicing embryos gently with laser light sheets.

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Huisken, Jan
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

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Huisken, J. (2012). Slicing embryos gently with laser light sheets. BioEssays: News and Reviews in Molecular, Cellular and Developmental Biology, 34(5), 406-411.

Cite as: https://hdl.handle.net/21.11116/0000-0001-08A8-9

Abstract

Light sheet microscopy is an easy to implement and extremely powerful alternative to established fluorescence imaging techniques such as laser scanning confocal, multi-photon and spinning disk microscopy. By illuminating the sample only with a thin slice of light, photo-bleaching is reduced to a minimum, making light sheet microscopy ideal for non-destructive imaging of fragile samples over extended periods of time. Millimeter-sized samples can be imaged rapidly with high resolution and high depth penetration. A large variety of instruments have been developed and optimized for a number of different samples: Bessel beams form thin light sheets for single cells, and selective plane illumination microscopy (SPIM) offers multi-view acquisition to image entire embryos with isotropic resolution. This review explains how light sheet microscopy involves a conceptually new microscope design and how it changes modern imaging in biology.