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Journal Article

The highly processive kinesin-8, Kip3, switches microtubule protofilaments with a bias towards the left

MPS-Authors
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Bormuth,  Volker
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

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Nitzsche,  Bert
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

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Ruhnow,  Felix
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

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Mitra,  Aniruddha
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

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Storch,  Marko
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

Rammner,  Burkhard
Max Planck Society;

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Howard,  Jonathon
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

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Diez,  Stefan
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

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Citation

Bormuth, V., Nitzsche, B., Ruhnow, F., Mitra, A., Storch, M., Rammner, B., et al. (2012). The highly processive kinesin-8, Kip3, switches microtubule protofilaments with a bias towards the left. Biophysical Journal, 103(1 (Letter)), 4-6.


Cite as: https://hdl.handle.net/21.11116/0000-0001-08C1-C
Abstract
Kinesin-1 motor proteins walk parallel to the protofilament axes of microtubules as they step from one tubulin dimer to the next. Is protofilament tracking an inherent property of processive kinesin motors, like kinesin-1, and what are the structural determinants underlying protofilament tracking? To address these questions, we investigated the tracking properties of the processive kinesin-8, Kip3. Using in vitro gliding motility assays, we found that Kip3 rotates microtubules counterclockwise around their longitudinal axes with periodicities of ∼1 μm. These rotations indicate that the motors switch protofilaments with a bias toward the left. Molecular modeling suggests 1), that the protofilament switching may be due to kinesin-8 having a longer neck linker than kinesin-1, and 2), that the leftward bias is due the asymmetric geometry of the motor neck linker complex.