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Journal Article

Fluorescence imaging of single Kinesin motors on immobilized microtubules.

MPS-Authors
/persons/resource/persons219334

Korten,  Till
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

/persons/resource/persons219491

Nitzsche,  Bert
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

/persons/resource/persons219179

Gell,  Christopher
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

/persons/resource/persons219596

Ruhnow,  Felix
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

/persons/resource/persons219376

Leduc,  Cecile
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

/persons/resource/persons219112

Diez,  Stefan
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

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Citation

Korten, T., Nitzsche, B., Gell, C., Ruhnow, F., Leduc, C., & Diez, S. (2011). Fluorescence imaging of single Kinesin motors on immobilized microtubules. Methods in Molecular Biology (Clifton, N.J.), 783, 121-137.


Cite as: https://hdl.handle.net/21.11116/0000-0001-0A14-E
Abstract
Recent developments in optical microscopy and nanometer tracking have greatly improved our understanding of cytoskeletal motor proteins. Using fluorescence microscopy, dynamic interactions are now routinely observed in vitro on the level of single molecules mainly using a geometry, where fluorescently labeled motors move on surface-immobilized filaments. In this chapter, we review recent methods related to single-molecule kinesin motility assays. In particular, we aim to provide practical advice on: how to set up the assays, how to acquire high-precision data from fluorescently labeled kinesin motors and attached quantum dots, and how to analyze data by nanometer tracking.