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Haematopoietic stem cell differentiation promotes the release of prominin-1/CD133-containing membrane vesicles-a role of the endocytic-exocytic pathway

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Bauer,  Nicola
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

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Wilsch-Bräuninger,  Michaela
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

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Fonseca,  Ana-Violeta
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

Strauss,  Doreen
Max Planck Society;

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Freund,  Daniel
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

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Thiele,  Christoph
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

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Huttner,  Wieland B.
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

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Corbeil,  Denis
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

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Citation

Bauer, N., Wilsch-Bräuninger, M., Karbanová, J., Fonseca, A.-V., Strauss, D., Freund, D., et al. (2011). Haematopoietic stem cell differentiation promotes the release of prominin-1/CD133-containing membrane vesicles-a role of the endocytic-exocytic pathway. EMBO Molecular Medicine, 3(7), 398-409.


Cite as: https://hdl.handle.net/21.11116/0000-0001-0A22-E
Abstract
The differentiation of stem cells is a fundamental process in cell biology and understanding its mechanism might open a new avenue for therapeutic strategies. Using an ex vivo co-culture system consisting of human primary haematopoietic stem and progenitor cells growing on multipotent mesenchymal stromal cells as a feeder cell layer, we describe here the exosome-mediated release of small membrane vesicles containing the stem and cancer stem cell marker prominin-1 (CD133) during haematopoietic cell differentiation. Surprisingly, this contrasts with the budding mechanism underlying the release of this cholesterol-binding protein from plasma membrane protrusions of neural progenitors. Nevertheless, in both progenitor cell types, protein-lipid assemblies might be the essential structural determinant in the release process of prominin-1. Collectively, these data support the concept that prominin-1-containing lipid rafts may host key determinants necessary to maintain stem cell properties and their quantitative reduction or loss may result in cellular differentiation.