In vitro evolution and analysis of HIV-1 LTR-specific recombinases

Buchholz, Frank; Hauber, Joachim

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In vitro evolution and analysis of HIV-1 LTR-specific recombinases

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Buchholz, Frank
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

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Buchholz, F., & Hauber, J. (2011). In vitro evolution and analysis of HIV-1 LTR-specific recombinases. Methods (San Diego, Calif.), 53(1), 102-109.

Cite as: https://hdl.handle.net/21.11116/0000-0001-0A3A-4

Abstract

Current antiretroviral therapies would greatly benefit from the concurrent removal of integrated HIV-1 proviral DNA from the patient's cells. In this review, we describe an experimental strategy that allowed the engineering and functional analysis of a HIV-1 LTR-specific recombinase (Tre-recombinase). We furthermore provide protocols that are utilized for the investigation of Tre's antiretroviral activity in infected tissue cultures as well as in infected humanized Rag2(-/-)γc(-/-) mice.