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Journal Article

Laser ablation of the microtubule cytoskeleton: setting up and working with an ablation system.

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Maghelli,  Nicola
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

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Tolic-Norrelykke,  Iva M.
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

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Citation

Maghelli, N., & Tolic-Norrelykke, I. M. (2011). Laser ablation of the microtubule cytoskeleton: setting up and working with an ablation system. Methods in Molecular Biology (Clifton, N.J.), 777, 261-271.


Cite as: https://hdl.handle.net/21.11116/0000-0001-0A42-A
Abstract
Laser ablation is a powerful tool that can be used to study a variety of biological mechanisms. Microscopes with high optical performances are nowadays available, and lasers that could be used to perform ablations have become accessible to every laboratory. Setting up a laser ablation system is a relatively straightforward task; however, it requires some basic knowledge of optics. We illustrate the fundamental components of the experimental setup and describe the most common pitfalls and difficulties encountered when designing, setting up, and working with a laser ablation system.