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CDK2AP1/DOC-1 is a bona fide subunit of the Mi-2/NuRD complex.

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Poser,  Ina
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

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Citation

Spruijt, C. G., Bartels, S. J. J., Brinkman, A. B., Tjeertes, J. V., Poser, I., Stunnenberg, H. G., et al. (2010). CDK2AP1/DOC-1 is a bona fide subunit of the Mi-2/NuRD complex. Molecular BioSystems, 6(9), 1700-1706.


Cite as: http://hdl.handle.net/21.11116/0000-0001-0B2D-2
Abstract
The Mi-2/NuRD (NUcleosome Remodeling and histone Deacetylase) chromatin remodeling complex is a large heterogeneous multiprotein complex associated with transcriptional repression. Here we apply a SILAC based quantitative proteomics approach to show that all known Mi-2/NuRD complex subunits co-purify with Cyclin Dependent Kinase 2 Associated Protein1 (CDK2AP1), also known as Deleted in Oral Cancer 1 (DOC-1). DOC-1 displays in vitro binding affinity for methylated DNA as part of the meCpG binding MBD2/NuRD complex. In luciferase reporter assays, DOC-1 is a potent repressor of transcription. Finally, immunofluorescence experiments reveal co-localization between MBD2 and DOC-1 in mouse NIH-3T3 nuclei. Collectively, these results indicate that DOC-1 is a bona fide subunit of the Mi-2/NuRD chromatin remodeling complex.