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Correlative time-lapse imaging and electron microscopy to study abscission in HeLa cells

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Mäntler,  Jana
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

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Müller-Reichert,  Thomas
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

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Citation

Guizetti, J., Mäntler, J., Müller-Reichert, T., & Gerlich, D. W. (2010). Correlative time-lapse imaging and electron microscopy to study abscission in HeLa cells. In T. Müller-Reichert (Ed.), Electron microscopy of model systems (pp. 591-601). Amsterdam, Netherlands: Elsevier.


Cite as: https://hdl.handle.net/21.11116/0000-0001-0B47-4
Abstract
HeLa cells are widely used as a model system to study cell division. The last step of cell division, abscission, occurs at an about 1 μm wide intercellular bridge that connects the post-mitotic sister cells. Abscission often occurs long after ingression of the cleavage furrow, and no efficient methods to synchronize cells to this stage are available. Here, we have developed a correlative fluorescence time-lapse imaging and electron microscopic approach using Aclar sheets with engraved grid patterns. This grid pattern, leaving a negative imprint on thin-layer embedded samples, allows identification of cells selected from the time-lapse imaging for serial-section electron microscopy. This method facilitates the ultrastructural analysis of specific stages of abscission.