English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
Add to Basket
  Local TagsRelease HistoryDetailsSummary

Released
Journal Article

Functional and spatial regulation of mitotic centromere-associated kinesin by cyclin-dependent kinase 1.

MPS-Authors
/persons/resource/persons219170

Friel,  Claire
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

Martin,  Claudia
Max Planck Society;

/persons/resource/persons219249

Howard,  Jonathon
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

External Resource
No external resources are shared
Fulltext (restricted access)
There are currently no full texts shared for your IP range.
Fulltext (public)
There are no public fulltexts stored in PuRe
Supplementary Material (public)
There is no public supplementary material available
Citation

Sanhaji, M., Friel, C., Kreis, N.-N., Krämer, A., Martin, C., Howard, J., et al. (2010). Functional and spatial regulation of mitotic centromere-associated kinesin by cyclin-dependent kinase 1. Molecular and Cellular Biology, 30(11), 2594-2607.


Cite as: https://hdl.handle.net/21.11116/0000-0001-0B71-4
Abstract
Mitotic centromere-associated kinesin (MCAK) plays an essential role in spindle formation and in correction of improper microtubule-kinetochore attachments. The localization and activity of MCAK at the centromere/kinetochore are controlled by Aurora B kinase. However, MCAK is also abundant in the cytosol and at centrosomes during mitosis, and its regulatory mechanism at these sites is unknown. We show here that cyclin-dependent kinase 1 (Cdk1) phosphorylates T537 in the core domain of MCAK and attenuates its microtubule-destabilizing activity in vitro and in vivo. Phosphorylation of MCAK by Cdk1 promotes the release of MCAK from centrosomes and is required for proper spindle formation. Interfering with the regulation of MCAK by Cdk1 causes dramatic defects in spindle formation and in chromosome positioning. This is the first study demonstrating that Cdk1 regulates the localization and activity of MCAK in mitosis by directly phosphorylating the catalytic core domain of MCAK.