Laser microsurgery provides evidence for merotelic kinetochore attachments in 
fission yeast cells lacking Pcs1 or Clr4

Rumpf, Cornelia; Cipak, Lubos; Schleiffer, Alexander; Pidoux, Alison; Mechtler, Karl; Tolic-Norrelykke, Iva M.; Gregan, Juraj

Item

ITEM ACTIONSEXPORT

Add to Basket

Local TagsRelease HistoryDetailsSummary

Released

Journal Article

Laser microsurgery provides evidence for merotelic kinetochore attachments in fission yeast cells lacking Pcs1 or Clr4

MPS-Authors

Pidoux, Alison
Max Planck Society;

/persons/resource/persons219741

Tolic-Norrelykke, Iva M.
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

External Resource

No external resources are shared

Fulltext (restricted access)

There are currently no full texts shared for your IP range.

Fulltext (public)

There are no public fulltexts stored in PuRe

Supplementary Material (public)

There is no public supplementary material available

Citation

Rumpf, C., Cipak, L., Schleiffer, A., Pidoux, A., Mechtler, K., Tolic-Norrelykke, I. M., et al. (2010). Laser microsurgery provides evidence for merotelic kinetochore attachments in fission yeast cells lacking Pcs1 or Clr4. Cell Cycle (Georgetown, Tex.), 9(19), 3997-4004.

Cite as: https://hdl.handle.net/21.11116/0000-0001-0B9E-2

Abstract

In order to segregate chromosomes properly, the cell must prevent merotelic kinetochore attachment, an error that occurs when a single kinetochore is attached to microtubules emanating from both spindle poles. Merotelic kinetochore orientation represents a major mechanism of aneuploidy in mitotic mammalian cells and it is the primary mechanism of chromosome instability in cancer cells. Fission yeast mutants defective in putative microtubule-site clamp Pcs1/ Mde4 or Clr4/Swi6-dependent centromeric heterochromatin display high frequencies of lagging chromosomes during anaphase. Here, we developed an assay based on laser microsurgery to show that the stretched morphology of lagging kinetochores in pcs1Δ and clr4Δ mutant cells is due to merotelic attachment. We further show that Mde4 is regulated by Cdc2 and that Cdc2 activity prevents precocious localization of Mde4 to the metaphase spindle. Finally, we show that Pcs1/Mde4 complex shares similar features with the conserved kinetochore complex Spc24/Spc25 suggesting that these two complexes may occupy a similar functional niche.