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A toolkit for high-throughput, cross-species gene engineering in Drosophila

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Ejsmont,  Radoslaw K
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

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Sarov,  Mihail
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

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Winkler,  Sylke
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

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Lipinski,  Kamil A
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

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Tomancak,  Pavel
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

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Citation

Ejsmont, R. K., Sarov, M., Winkler, S., Lipinski, K. A., & Tomancak, P. (2009). A toolkit for high-throughput, cross-species gene engineering in Drosophila. Nature Methods, 6(6), 435-437.


Cite as: https://hdl.handle.net/21.11116/0000-0001-0CC4-5
Abstract
We generated two complementary genomic fosmid libraries for Drosophila melanogaster and Drosophila pseudoobscura that permit seamless modification of large genomic clones by high-throughput recombineering and direct transgenesis. The fosmid transgenes recapitulated endogenous gene expression patterns. These libraries, in combination with recombineering technology, will be useful to rescue mutant phenotypes, allow imaging of gene products in living flies and enable systematic analysis and manipulation of gene activity across species.