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Journal Article

Live cell multicolor imaging of lipid droplets with a new dye, LD540

MPS-Authors
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Spandl,  Johanna
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

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Peychl,  Jan
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

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Thiele,  Christoph
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

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Citation

Spandl, J., White, D. J., Peychl, J., & Thiele, C. (2009). Live cell multicolor imaging of lipid droplets with a new dye, LD540. Traffic, 10(11), 1579-1584.


Cite as: https://hdl.handle.net/21.11116/0000-0001-0D3F-C
Abstract
A lipophilic dye based on the Bodipy fluorophore, LD540, was developed for microscopic imaging of lipid droplets. In contrast to previous lipid droplet dyes, it can spectrally be resolved from both green and red fluorophores allowing multicolor imaging in both fixed and living cells. Its improved specificity, brightness and photostability support live cell imaging, which was used to demonstrate by two-color imaging lipid droplet motility along microtubules.