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Release of extracellular membrane vesicles from microvilli of epithelial cells is enhanced by depleting membrane cholesterol

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Marzesco,  Anne-Marie
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

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Wilsch-Bräuninger,  Michaela
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

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Dubreuil,  Veronique
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

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Janich,  Peggy
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

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Langenfeld,  Katja
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

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Thiele,  Christoph
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

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Huttner,  Wieland B.
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

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Corbeil,  Denis
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

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Citation

Marzesco, A.-M., Wilsch-Bräuninger, M., Dubreuil, V., Janich, P., Langenfeld, K., Thiele, C., et al. (2009). Release of extracellular membrane vesicles from microvilli of epithelial cells is enhanced by depleting membrane cholesterol. FEBS Letters, 583(5), 897-902.


Cite as: https://hdl.handle.net/21.11116/0000-0001-0D72-1
Abstract
We previously reported on the occurrence of prominin-1-carrying membrane vesicles that are released into body fluids from microvilli of epithelial cells. This release has been implicated in cell differentiation. Here we have characterized these vesicles released from the differentiated Caco-2 cells. We find that in these vesicles, prominin-1 directly interacts with membrane cholesterol and is associated with a membrane microdomain. The cholesterol depletion using methyl-b-cyclodextrin resulted in a marked increase in their release, and a dramatic change in the microvillar ultrastructure from a tubular shape to a ‘‘pearling” state, with multiple membrane constrictions, suggesting a role of membrane cholesterol in vesicle release from microvilli.