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Journal Article

Simplified validation of borderline hits of database searches

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Thomas,  Henrik
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

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Shevchenko,  Andrej
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

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Citation

Thomas, H., & Shevchenko, A. (2008). Simplified validation of borderline hits of database searches. PROTEOMICS, 8(20), 4173-4177.


Cite as: https://hdl.handle.net/21.11116/0000-0001-0E8F-0
Abstract
Along with unequivocal hits produced by matching multiple MS/MS spectra to database sequences, LC-MS/MS analysis often yields a large number of hits of borderline statistical confidence. To simplify their validation, we propose to use rapid de novo interpretation of all acquired MS/MS spectra and, with the help of a simple software tool, display the candidate sequences together with each database search hit. We demonstrate that comparing hit database sequences and independent de novo interpretations of the same MS/MS spectra assists in rapid examination of ambiguous matches.