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Journal Article

siRNA screening reveals JNK2 as an evolutionary conserved regulator of triglyceride homeostasis

MPS-Authors
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Grimard,  Vinciane
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

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Massier,  Julia
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

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Richter,  Doris
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

/persons/resource/persons219659

Schwudke,  Dominik
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

/persons/resource/persons219285

Kalaidzidis,  Yannis
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

/persons/resource/persons219150

Fava,  Eugenio
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

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Thiele,  Christoph
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

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Citation

Grimard, V., Massier, J., Richter, D., Schwudke, D., Kalaidzidis, Y., Fava, E., et al. (2008). siRNA screening reveals JNK2 as an evolutionary conserved regulator of triglyceride homeostasis. Journal of Lipid Research, 49(11), 2427-2440.


Cite as: https://hdl.handle.net/21.11116/0000-0001-0E93-A
Abstract
Lipid homeostasis is essential for proper function of cells and organisms. To unravel new regulators of this system, we developed a screening procedure, combining RNA interference in HeLa cells and TLC, which enabled us to monitor modifications of lipid composition resulting from short, interfering RNA knock-downs. We applied this technique to the analysis of 600 human kinases. Despite the occurrence of off-target effects, we identified JNK2 as a new player in triglyceride (TG) homeostasis and lipid droplet metabolism and, more specifically, in the regulation of lipolysis. Similar control of the level of TGs and lipid droplets was observed for its Schizosaccharomyces pombe homolog, Sty1, suggesting an evolutionary conserved function of mitogen-activated protein kinases in the regulation of lipid storage in eukaryotic cells.