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Journal Article

Versatile laser-based cell manipulator


Maghelli,  Nicola
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;


Tolic-Norrelykke,  Iva M.
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

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Maghelli, N., & Tolic-Norrelykke, I. M. (2008). Versatile laser-based cell manipulator. Journal of Biophotonics, 1(4), 299-309.

Cite as: https://hdl.handle.net/21.11116/0000-0001-0EC4-3
Here we describe a two-photon microscope and laser ablation setup combined with optical tweezers. We tested the setup on the fission yeast Schizosaccharomyces pombe, a commonly used model organism. We show that long-term imaging can be achieved without significant photo-bleaching or damage of the sample. The setup can precisely ablate sub-micrometer structures, such as microtubules and mitotic spindles, inside living cells, which remain viable after the manipulation. Longer exposure times lead to ablation, while shorter exposures lead to photo-bleaching of the target structure. We used optical tweezers to trap intracellular particles and to displace the cell nucleus. Two-photon fluorescence imaging of the manipulated cell can be performed simultaneously with trapping. The combination of techniques described here may help to solve a variety of problems in cell biology, such as positioning of organelles and the forces exerted by the cytoskeleton.