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Contributions of galectin-3 and -9 to epithelial cell adhesion analyzed by single cell force spectroscopy

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/persons/resource/persons219168

Friedrichs,  Jens
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

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Torkko,  Juha M.
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

/persons/resource/persons219233

Helenius,  Jonne
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

/persons/resource/persons219172

Fullekrug,  Joachim
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

/persons/resource/persons219671

Simons,  Kai
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

/persons/resource/persons219420

Manninen,  Aki
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

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Citation

Friedrichs, J., Torkko, J. M., Helenius, J., Teravainen, T. P., Fullekrug, J., Muller, D. J., et al. (2007). Contributions of galectin-3 and -9 to epithelial cell adhesion analyzed by single cell force spectroscopy. Journal of Biological Chemistry, 282(40), 29375-29383.


Cite as: https://hdl.handle.net/21.11116/0000-0001-0F14-9
Abstract
Galectins are widely expressed in epithelial tissues and have been implicated in a variety of cellular processes, including adhesion and polarization. Here we studied the contributions of galectins in cell adhesion and cyst formation of Madin-Darby canine kidney cells. Quantitative single cell force spectroscopy and standard adhesion assays were employed to study both early (<2 min) and long term (90 min) adhesion of cells to different extracellular matrix components. Inhibitors were used to examine the contribution of integrins and galectins in general and RNA interference to specifically address the role of two abundantly expressed galectins, galectin-3 and -9. We found that both galectin-3 and -9 were required for optimal long term cell adhesion to both collagen I and laminin-111. Early adhesion to laminin was found to be integrin-independent and was instead mediated by carbohydrate interactions and galectin-3 and -9. The opposite was observed for early adhesion to collagen. Although similar, the contributions of galectin-3 and -9 to adhesion appeared to be by distinct processes. These defects in adhesion of the two galectin knockdown cell lines may underlie the epithelial phenotypes observed in the cyst assays. Our findings emphasize the complex regulation of epithelial cell functions by galectins.