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Journal Article

Detergent resistance as a tool in membrane research

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Lingwood,  Daniel
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

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Simons,  Kai
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

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Citation

Lingwood, D., & Simons, K. (2007). Detergent resistance as a tool in membrane research. Nature Protocols, 2(9), 2159-2165.


Cite as: https://hdl.handle.net/21.11116/0000-0001-0F1E-F
Abstract
The biological membrane is a complicated matrix wherein different lipid environments are thought to exist. The more ordered or raft environment has been perceived biochemically accessible via its relative resistance to detergent. This paper outlines the protocols developed in our laboratory for the analysis of such detergent-resistant membranes (DRMs). We stress the fact that DRMs are artifactual in nature and should not be equivocated to lipid rafts, their usefulness being limited to assigning raft-association potential most convincingly when changes in DRM composition are induced by biochemically/physiologically relevant events. These protocols are completed in 1–2 d.