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Journal Article

The saliva proteome of the blood-feeding insect Triatoma infestans is rich in platelet-aggregation inhibitors


Shevchenko,  Andrej
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

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Charneau, S., Junqueira, M., Costa, C. M., Pires, D. L., Fernandes, E. S., Bussacos, A. C., et al. (2007). The saliva proteome of the blood-feeding insect Triatoma infestans is rich in platelet-aggregation inhibitors. International Journal of Mass Spectrometry, 268(2-3), 265-276.

Cite as: https://hdl.handle.net/21.11116/0000-0001-0FBA-E
The saliva of the bloodsucking bug Triatoma infestans vector of Chagas disease contains an anti-hemostatic molecular cocktail that prevents
coagulation, vasoconstriction and platelet aggregation in a vertebrate prey. In order to characterize T. infestans saliva proteome, we separated the
secreted saliva by two-dimensional gel electrophoresis (2-DE). More than 200 salivary proteins were detected on the 2-DE map, mainly in the
alkaline region. By nanoLC–MS/MS analysis using a LTQ–Orbitrap equipment followed by a combination of conventional and sequence-similarity
searches, we identified 58 main protein spots. Most of such proteins possess potential blood-feeding associated functions, particularly anti-platelet
aggregation proteins belonging to lipocalin and apyrase families. The saliva protein composition indicates a highly specific molecular mechanism
of early response to platelet aggregation. This first proteome analysis of the T. infestans secreted saliva provides a basis for a better understanding
of this fluid protein composition highly directed to counterpart hemostasis of the prey, thus promoting the bug’s blood-feeding.