The saliva proteome of the blood-feeding insect Triatoma infestans is rich in 
platelet-aggregation inhibitors

Charneau, Sébastien; Junqueira, Magno; Costa, Camila M.; Pires, Daniele L.; Fernandes, Ellen S.; Bussacos, Ana C.; Sousa, Marcelo V.; Ricart, Carlos André O.; Shevchenko, Andrej; Teixeira, Antonio R.L.

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The saliva proteome of the blood-feeding insect Triatoma infestans is rich in platelet-aggregation inhibitors

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Shevchenko, Andrej
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

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引用

Charneau, S., Junqueira, M., Costa, C. M., Pires, D. L., Fernandes, E. S., Bussacos, A. C., Sousa, M. V., Ricart, C. A. O., Shevchenko, A., & Teixeira, A. R. (2007). The saliva proteome of the blood-feeding insect Triatoma infestans is rich in platelet-aggregation inhibitors. International Journal of Mass Spectrometry, 268(2-3), 265-276.

引用: https://hdl.handle.net/21.11116/0000-0001-0FBA-E

要旨

The saliva of the bloodsucking bug Triatoma infestans vector of Chagas disease contains an anti-hemostatic molecular cocktail that prevents
coagulation, vasoconstriction and platelet aggregation in a vertebrate prey. In order to characterize T. infestans saliva proteome, we separated the
secreted saliva by two-dimensional gel electrophoresis (2-DE). More than 200 salivary proteins were detected on the 2-DE map, mainly in the
alkaline region. By nanoLCMS/MS analysis using a LTQOrbitrap equipment followed by a combination of conventional and sequence-similarity
searches, we identified 58 main protein spots. Most of such proteins possess potential blood-feeding associated functions, particularly anti-platelet
aggregation proteins belonging to lipocalin and apyrase families. The saliva protein composition indicates a highly specific molecular mechanism
of early response to platelet aggregation. This first proteome analysis of the T. infestans secreted saliva provides a basis for a better understanding
of this fluid protein composition highly directed to counterpart hemostasis of the prey, thus promoting the bugs blood-feeding.