DNA translocation and loop formation mechanism of chromatin remodeling by 
SWI/SNF and RSC

Zhang, Yongli; Smith, Corey L; Saha, Anjanabha; Grill, Stephan W; Mihardja, Shirley; Smith, Steven B; Cairns, Bradley R; Peterson, Craig L; Bustamante, Carlos

Item

ITEM ACTIONSEXPORT

Add to Basket

Local TagsRelease HistoryDetailsSummary

Released

Journal Article

DNA translocation and loop formation mechanism of chromatin remodeling by SWI/SNF and RSC

MPS-Authors

/persons/resource/persons145692

Grill, Stephan W
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

External Resource

No external resources are shared

Fulltext (restricted access)

There are currently no full texts shared for your IP range.

Fulltext (public)

There are no public fulltexts stored in PuRe

Supplementary Material (public)

There is no public supplementary material available

Citation

Zhang, Y., Smith, C. L., Saha, A., Grill, S. W., Mihardja, S., Smith, S. B., et al. (2006). DNA translocation and loop formation mechanism of chromatin remodeling by SWI/SNF and RSC. Molecular Cell, 24(4), 559-568.

Cite as: https://hdl.handle.net/21.11116/0000-0001-1038-E

Abstract

ATP-dependent chromatin-remodeling complexes (remodelers) modulate gene transcription by regulating the accessibility of highly packaged genomic DNA. However, the molecular mechanisms involved at the nucleosomal level in this process remain controversial. Here, we monitor the real-time activity of single ySWI/SNF or RSC complexes on single, stretched nucleosomal templates under tensions above 1 pN forces. We find that these remodelers can translocate along DNA at rates of approximately 13 bp/s and generate forces up to approximately 12 pN, producing DNA loops of a broad range of sizes (20-1200 bp, average approximately 100 bp) in a nucleosome-dependent manner. This nucleosome-specific activity differs significantly from that on bare DNA observed under low tensions and suggests a nucleosome-remodeling mechanism through intranucleosomal DNA loop formation. Such loop formation may provide a molecular basis for the biological functions of remodelers.