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Enzymatically prepared RNAi libraries

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Buchholz,  Frank
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

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Kittler,  Ralf
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

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Slabicki,  Mikolaj
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

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Theis,  Mirko
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

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Citation

Buchholz, F., Kittler, R., Slabicki, M., & Theis, M. (2006). Enzymatically prepared RNAi libraries. Nature Methods, 3(9), 696-700.


Cite as: http://hdl.handle.net/21.11116/0000-0001-1040-4
Abstract
Large-scale RNA interference (RNAi) screens in mammalian cells have mainly used synthetic small interfering RNA (siRNA) or short hairpin RNA (shRNA) libraries. The RNAi triggers for both of these approaches were designed with algorithm-based predictions to identify single sequences for mRNA knockdown. Alternatives to these approaches have recently been developed using enzymatic methods. Here we describe the concepts of enzymatically prepared shRNA and siRNA libraries, and discuss their strengths and limitations.