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Journal Article

Proteomics of early zebrafish embryos

MPS-Authors
/persons/resource/persons219390

Link,  Vinzenz
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

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Shevchenko,  Andrej
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

/persons/resource/persons219231

Heisenberg,  Carl-Philipp
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

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Citation

Link, V., Shevchenko, A., & Heisenberg, C.-P. (2006). Proteomics of early zebrafish embryos. BMC Developmental Biology, 6(1), 1-9.


Cite as: https://hdl.handle.net/21.11116/0000-0001-1096-3
Abstract
Background Zebrafish (D. rerio) has become a powerful and widely used model system for the analysis of vertebrate embryogenesis and organ development. While genetic methods are readily available in zebrafish, protocols for two dimensional (2D) gel electrophoresis and proteomics have yet to be developed. Results As a prerequisite to carry out proteomic experiments with early zebrafish embryos, we developed a method to efficiently remove the yolk from large batches of embryos. This method enabled high resolution 2D gel electrophoresis and improved Western blotting considerably. Here, we provide detailed protocols for proteomics in zebrafish from sample preparation to mass spectrometry (MS), including a comparison of databases for MS identification of zebrafish proteins. Conclusion The provided protocols for proteomic analysis of early embryos enable research to be taken in novel directions in embryogenesis.