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Journal Article

zvg-8, a gene required for spindle positioning in C-elegans, encodes a doublecortin-related kinase that promotes microtubule assembly

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Kirkham,  M.
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

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Pozniakowski,  A.
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

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Hyman,  A. A.
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

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Citation

Gonczy, P., Bellanger, J. M., Kirkham, M., Pozniakowski, A., Baumer, K., Phillips, J. B., et al. (2001). zvg-8, a gene required for spindle positioning in C-elegans, encodes a doublecortin-related kinase that promotes microtubule assembly. Developmental Cell, 1(3), 363-375.


Cite as: https://hdl.handle.net/21.11116/0000-0001-13F8-2
Abstract
Proper spindle positioning is essential for spatial control of cell division. Here, we show that zyg-8 plays a key role in spindle positioning during asymmetric division of one-cell stage C. elegans embryos by promoting microtubule assembly during anaphase. ZYG-8 harbors a kinase domain and a domain related to Doublecortin, a microtubule-associated protein (MAP) affected in patients with neuronal migration disorders. Sequencing of zyg-8 mutant alleles demonstrates that both domains are essential for function. ZYG-8 binds to microtubules in vitro, colocalizes with microtubules in vivo, and promotes stabilization of microtubules to drug or cold depolymerization in COS-7 cells. Our findings demonstrate that ZYG-8 is a MAP crucial for proper spindle positioning in C. elegans, and indicate that the function of the Doublecortin domain in modulating microtubule dynamics is conserved across metazoan evolution.