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Journal Article

Human leukocyte interferon produced by E. coli is biologically active


Seeburg,  Peter H.
Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society;

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Goeddel, D. V., Yelverton, E., Ullrich, A., Heyneker, H. L., Miozzari, G., Holmes, W., et al. (1980). Human leukocyte interferon produced by E. coli is biologically active. Nature, 287(5781), 411-416. Retrieved from http://www.nature.com/nature/journal/v287/n5781/abs/287411a0.html.

Cite as: http://hdl.handle.net/21.11116/0000-0001-19A0-E
A human leukocyte interferon cDNA was enzymatically synthesized, inserted into the vector pBR322, and cloned in Escherichia coli. The DNA sequence codes for a 23-amino acid signal peptide followed by an interferon polypeptide of 165 amino acids. An expression plasmid was constructed which permits the synthesis in E. coli of 2.5 x 10(8) units of interferon per litre of culture. This LeIF protected squirrel monkeys from lethal encephalomyocarditis virus infection.