Juxtasomal Biocytin Labeling to Study the Structure-function Relationship of 
Individual Cortical Neurons

Narayanan, RT; Mohan, H; Broersen, R; de Haan, R; Pienemann, AW; de Kock, CPJ

doi:10.3791/51359

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Juxtasomal Biocytin Labeling to Study the Structure-function Relationship of Individual Cortical Neurons

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Narayanan, R., Mohan, H., Broersen, R., de Haan, R., Pienemann, A., & de Kock, C. (2014). Juxtasomal Biocytin Labeling to Study the Structure-function Relationship of Individual Cortical Neurons. Journal of Visualized Experiments, 2014: 84, pp. 1-9. doi:10.3791/51359.

Cite as: https://hdl.handle.net/21.11116/0000-0001-2C05-9

Abstract

To understand the structure of neuronal networks, functional and morphological characterization of individual neurons is a necessity. Here, we demonstrate juxtasomal biocytin labeling, which allows electrophysiological recordings in the extracellular configuration, yet maintaining the ability to intracellularly label the neuron for post hoc reconstruction of dendritic and axonal architecture.