English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT

Released

Journal Article

Vesicle adhesion and fusion studied by small-angle x-ray scattering.

MPS-Authors
/persons/resource/persons220498

Komorowski,  K.
Department of Neurobiology, MPI for Biophysical Chemistry, Max Planck Society;

/persons/resource/persons132892

Yavuz,  H.
Department of Neurobiology, MPI for Biophysical Chemistry, Max Planck Society;

/persons/resource/persons15266

Jahn,  R.
Department of Neurobiology, MPI for Biophysical Chemistry, Max Planck Society;

External Ressource
No external resources are shared
Fulltext (public)

2583545.pdf
(Publisher version), 2MB

Supplementary Material (public)

2583545_Suppl_1.pdf
(Supplementary material), 3MB

2583545_Suppl_2.pdf
(Supplementary material), 4MB

Citation

Komorowski, K., Salditt, A., Xu, Y., Yavuz, H., Brennich, M., Jahn, R., et al. (2018). Vesicle adhesion and fusion studied by small-angle x-ray scattering. Biophysical Journal, 114(8), 1908-1920. doi:10.1016/j.bpj.2018.02.040.


Cite as: http://hdl.handle.net/21.11116/0000-0001-3BED-3
Abstract
We have studied the adhesion state (also denoted by docking state) of lipid vesicles as induced by the divalent ions Ca2+ or Mg2+ at well-controlled ion concentration, lipid composition, and charge density. The bilayer structure and the interbilayer distance in the docking state were analyzed by small-angle x-ray scattering. A strong adhesion state was observed for DOPC:DOPS vesicles, indicating like-charge attraction resulting from ion correlations. The observed interbilayer separations of ∼1.6 nm agree quantitatively with the predictions of electrostatics in the strong coupling regime. Although this phenomenon was observed when mixing anionic and zwitterionic (or neutral) lipids, pure anionic membranes (DOPS) with highest charge density σ resulted in a direct phase transition to a multilamellar state, which must be accompanied by rupture and fusion of vesicles. To extend the structural assay toward protein-controlled docking and fusion, we have characterized reconstituted N-ethylmaleimide-sensitive factor attachment protein receptors in controlled proteoliposome suspensions by small-angle x-ray scattering.