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Journal Article

Simple paired heavy- and light-chain antibody repertoire sequencing using endoplasmic reticulum microsomes

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Konthur,  Zoltán
Zoltán Konthur, Biomolekulare Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society;

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Citation

Devulapally, P. R., Bürger, J., Mielke, T., Konthur, Z., Lehrach, H., Yaspo, M.-L., et al. (2018). Simple paired heavy- and light-chain antibody repertoire sequencing using endoplasmic reticulum microsomes. Genome Medicine, 10: 34. doi:10.1186/s13073-018-0542-5.


Cite as: http://hdl.handle.net/21.11116/0000-0001-4018-C
Abstract
Existing methods for paired antibody heavy- and light-chain repertoire sequencing rely on specialized equipment and are limited by their commercial availability and high costs. Here, we report a novel simple and cost-effective emulsion-based single-cell paired antibody repertoire sequencing method that employs only basic laboratory equipment. We performed a proof-of-concept using mixed mouse hybridoma cells and we also showed that our method can be used for discovery of novel antigen-specific monoclonal antibodies by sequencing human CD19+ B cell IgM and IgG repertoires isolated from peripheral whole blood before and seven days after Td (Tetanus toxoid/Diphtheria toxoid) booster immunization. We anticipate broad applicability of our method for providing insights into adaptive immune responses associated with various diseases, vaccinations, and cancer immunotherapies.