Two-colour serial femtosecond crystallography dataset from 
gadoteridol-derivatized lysozyme for MAD phasing

Gorel, Alexander; Motomura, Koji; Fukuzawa, Hironobu; Doak, R. Bruce; Grünbein, Marie Luise; Hilpert, Mario; Inoue, Ichiro; Kloos, Marco; Nass Kovács, Gabriela; Nango, Eriko; Nass, Karol; Roome, Christopher M.; Shoeman, Robert L.; Tanaka, Rie; Tono, Kensuke; Foucar, Lutz; Joti, Yasumasa; Yabashi, Makina; Iwata, So; Ueda, Kiyoshi; Barends, Thomas R. M.; Schlichting, Ilme

doi:10.1038/sdata.2017.188

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Two-colour serial femtosecond crystallography dataset from gadoteridol-derivatized lysozyme for MAD phasing

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Gorel, Alexander
Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society;

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Doak, R. Bruce
Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society;

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Grünbein, Marie Luise
Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society;

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Hilpert, Mario
Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society;

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Kloos, Marco
Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society;

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Nass Kovács, Gabriela
Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society;

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Nass, Karol
Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society;

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Roome, Christopher M.
Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society;

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Shoeman, Robert L.
Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society;

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Foucar, Lutz
Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society;

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Barends, Thomas R. M.
Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society;

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Schlichting, Ilme
Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society;

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https://doi.org/10.1038/sdata.2017.188
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Citation

Gorel, A., Motomura, K., Fukuzawa, H., Doak, R. B., Grünbein, M. L., Hilpert, M., et al. (2017). Two-colour serial femtosecond crystallography dataset from gadoteridol-derivatized lysozyme for MAD phasing. Scientific Data, 4: 170188, pp. 1-11. doi:10.1038/sdata.2017.188.

Cite as: https://hdl.handle.net/21.11116/0000-0001-3EE5-8

Abstract

We provide a detailed description of a gadoteridol-derivatized lysozyme (gadolinium lysozyme) two-colour serial femtosecond crystallography (SFX) dataset for multiple wavelength anomalous dispersion (MAD) structure determination. The data was collected at the Spring-8 Angstrom Compact free-electron LAser (SACLA) facility using a two-colour double-pulse beam to record two diffraction patterns simultaneously in one diffraction image. Gadolinium lysozyme was chosen as a well-established model system that has a very strong anomalous signal. Diffraction patterns from gadolinium lysozyme microcrystals were recorded to a resolution of 1.9 Å in both colours. This dataset is publicly available through the Coherent X-ray Imaging Data Bank (CXIDB) as a resource for algorithm development.