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Visualizing single-cell secretion dynamics with single protein sensitivity

MPG-Autoren
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McDonald,  Matthew Paul
Sandoghdar Division, Max Planck Institute for the Science of Light, Max Planck Society;

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Gemeinhardt,  André
Sandoghdar Division, Max Planck Institute for the Science of Light, Max Planck Society;

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König,  Katharina
Sandoghdar Division, Max Planck Institute for the Science of Light, Max Planck Society;
International Max Planck Research School, Max Planck Institute for the Science of Light, Max Planck Society;

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Piliarik,  Marek
Sandoghdar Division, Max Planck Institute for the Science of Light, Max Planck Society;

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Sandoghdar,  Vahid
Sandoghdar Division, Max Planck Institute for the Science of Light, Max Planck Society;
Max-Planck-Zentrum für Physik und Medizin, Max Planck Institute for the Science of Light, Max Planck Society;

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Zitation

McDonald, M. P., Gemeinhardt, A., König, K., Piliarik, M., Schaffer, S., Völkl, S., et al. (2018). Visualizing single-cell secretion dynamics with single protein sensitivity. Nano Letters, 18, 513-519. doi:10.1021/acs.nanolett.7b04494.


Zitierlink: https://hdl.handle.net/21.11116/0000-0001-55D9-B
Zusammenfassung
Cellular secretion of proteins into the extracellular environment is an essential mediator of critical biological mechanisms, including cell-to-cell communication, immunological response, targeted delivery, and differentiation. Here, we report a novel methodology that allows for the real-time detection and imaging of single unlabeled proteins that are secreted from individual living cells. This is accomplished via interferometric detection of scattered light (iSCAT) and is demonstrated with Laz388 cells, an Epstein Barr virus (EBV)-transformed B cell line. We find that single Laz388 cells actively secrete IgG antibodies at a rate of the order of 100 molecules per second. Intriguingly, we also find that other proteins and particles spanning ca. 100 kDa-1 MDa are secreted from the Laz388 cells in tandem with IgG antibody release, likely arising from EBV-related viral proteins. The technique is general and, as we show, can also be applied to studying the lysate of a single cell. Our results establish label-free iSCAT imaging as a powerful tool for studying the real-time exchange between cells and their immediate environment with single-protein sensitivity.