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Cell type-specific 3D structure and in vivo function of rat vibrissal sensory and motor cortex

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Narayanan,  RT
Former Research Group Computational Neuroanatomy, Max Planck Institute for Biological Cybernetics, Max Planck Society;
Max Planck Institute for Biological Cybernetics, Max Planck Society;

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Egger,  R
Former Research Group Computational Neuroanatomy, Max Planck Institute for Biological Cybernetics, Max Planck Society;
Max Planck Institute for Biological Cybernetics, Max Planck Society;

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Oberlaender,  M
Former Research Group Computational Neuroanatomy, Max Planck Institute for Biological Cybernetics, Max Planck Society;
Max Planck Institute for Biological Cybernetics, Max Planck Society;

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Citation

Narayanan, R., De Kock, C., Baltruschat, L., Mohan, H., Egger, R., Sakmann, B., et al. (2013). Cell type-specific 3D structure and in vivo function of rat vibrissal sensory and motor cortex. Poster presented at Bernstein Conference 2013, Tübingen, Germany.


Cite as: https://hdl.handle.net/21.11116/0000-0001-51B2-A
Abstract
Despite a long tradition in reconstructing neurons, the tracing of complete 3D axon morphologies still represents one major challenge in neuroscience research. In the present study, we labelled individual neurons throughout all layers of rat somatosensory and motor cortex with biocytin using an in vivo juxtasomal labelling approach. These neurons were reconstructed for their axon and dendrite morphologies by a semi-automated tracing pipeline and then positioned in a standard cortex reference frame to determine potential dendrite-axon overlap between neurons to create anatomically realistic model of the cortical circuitry. Previously we showed that the dendritic length and the number of thalamocortical synapses are key to predict the spiking behaviour of individual neurons in vivo. Here, additionally we show that all cell types in the rat vibrissal cortex have very specific local and long range innervation profiles. For e.g. we found that spiny stellate in the main input layer 4 are presynaptic to all cortical layers, whereas thick tufted neurons in the main output layer 5 are postsynaptic to all layers. Secondly, we found that axons of individual neurons show location and cell type-specific bouton density profiles. Thirdly, we found that all sensory neurons project axons beyond their principal column. Hence, most cell types receive more than 50% of their intracortical input from surrounding columns. Thus, for the first time, we provide clear evidence of multi-columnar processing. However, it remains open, whether some of these principles may be generalizable to other cortical areas as well. Consequently, we determined in vivo spiking frequencies, dendritic and axon projection patterns for cell types located within the vibrissal part of the primary motor cortex. We will demonstrate similarities, but also substantial organizational differences between the sensory and motor areas involved in processing whisker-evoked excitation.